Main research area:
PE5_7 - Biomaterials, biomaterials synthesis
Secondary research area:
PE8_8 - Materials engineering (metals, ceramics, polymers, composites, etc.)
Mineral scaffolds, Bacterial cellulose, Electrospinning, Ferromagnetic nanoparticles, Osteoinductive properties
The aim of the present project proposal consist in the developing of new mineral scaffolds by employing two different types of templates, followed by ferromagnetic nanoparticles loading in order to improve the biological response of the final mineral material. The first template used for the fabrication of calcium phosphate based scaffolds is represented by bacterial cellulose membranes produced extracellularly by microorganisms in our laboratory, while the other one is artificially obtained by electrospinning polymeric solutions in the form of nonwoven 3D networks. The hybrid composite structures made of organic template and calcium phosphates are subjected to thermal treatments in various conditions in order to completely remove the sacrificial polymers, enhance the degree of crystallinity for the mineral phases and provide consolidated self-sustained architectures with appropriate structural and morphological characteristics for bone reconstruction. The next step focuses on the improvement of the biological properties of the resulted mineral scaffolds by loading them with ferromagnetic nanoparticles prepared by wet-chemistry methods, with important repercussions on the osteoinductive properties of the biomaterial. The biological behaviour of the obtained smart scaffolds will be assessed by in vitro tests, since this type of experiments are both ethical and economical. Thus, bioactivity will be studied by examining the response of the mineral scaffolds decorated with ferromagnetic nanoparticles after immersion in simulated body fluids, while the biocompatibility of the composite 3D architectures will be investigated by standard assays conducted on cell cultures. Not least, the project aims to develop a laboratory experimental demonstrator starting from a formulated product concept, whose functionality will be proved by preclinical in vivo evaluation.
The scope of the project consist in the design and development of smart scaffolds starting from biocellulose 3D architecture or artificial electrospun templates, as well as the validation of their suitability for the field of hard tissue repair and regeneration.
1. Obtaining of mineral scaffolds decorated with ferromagnetic nanoparticles
2. Complex characterization of smart scaffolds by specific techniques
3. Writing the research report of each step
4. Dissemination of the research results by writing and submitting articles for publication in scientific journals and national patent applications, as well as by participating at international conferences
5. Increasing the capacity of young researchers to coordinate teams and implement their own research project
6. Growing the international visibility of Romanian young researchers
V. Dinca, A. Mocanu, G. Isopencu, C. Busuioc, S. Brajnicov, A. Vlad, M. Icriverzi, A. Roseanu, M. Dinescu, M. Stroescu, A. Stoica-Guzun, M. Suchea, Biocompatible Pure ZnO Nanoparticles-3D Bacterial Cellulose Biointerfaces with Antibacterial Properties, Arabian Journal of Chemistry (2018) -Read article online
C. Busuioc, C.D. Ghitulica, A. Stoica, M. Stroescu, G. Voicu, V. Ionita, L. Averous, S.I. Jinga, Calcium Phosphates Grown on Bacterial Cellulose Template, Ceramics International 44 (2018) 9433–9441
C. Busuioc, A. Stoica, M. Stroescu, S.I. Jinga, Influence of Processing Parameters on the Properties of Calcium Phosphates Based Scaffolds, 12th International Conference on Phyisics of Advanced Materials, September 2018, Heraklion, Greece (poster presentation).
C. Busuioc, A. Stoica, M. Stroescu, S.I. Jinga, Template Assisted Synthesis of Calcium Phosphates - BaTiO3 Composite Scaffolds, 14th International Conference on Advanced Nanomaterials, July 2018, Aveiro, Portugal (poster presentation).